Synergistic effects of thalidomide and poly(ADP-ribose) polymerase inhibition on type II collagen-induced arthritis in mice

被引：28

作者：

Kroger, H

Miesel, R

Dietrich, A

Ohde, M

Rajnavolgyi, E

Ockenfels, H

机构：

[1] UNIV OTAGO, DEPT BIOCHEM, DUNEDIN, NEW ZEALAND

[2] UNIV ULM, DEPT PHYSIOL CHEM, W-7900 ULM, GERMANY

来源：

INFLAMMATION | 1996年 / 20卷 / 02期

关键词：

D O I：

10.1007/BF01487406

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The present study investigates synergistic effects of the TNF-alpha inhibitor thalidomide and the poly(ADP-ribose) polymerase (PARP)-inhibitor nicotinic acid amide (NAA) in male DBA/1 hybrid mice suffering from type 11 collagen-induced arthritis. Parameters including the arthritis index, chemiluminescence and anti-collagen antibody titers were used for the assessment of disease activity: The disease courses demonstrated clearly an inhibitory effect of thalidomide. NAA inhibited established collagen arthritis in a dose-dependent manner. The combined application of thalidomide and NAA caused a powerful synergistic inhibition of arthritis. Furthermore, thalidomide and NAA were tested ex vivo for their inhibition of the NADPH oxidase-dependent generation of reactive oxygen species by activated neutrophils and monocytes in unseparated human blood. Our data show that type II collagen-induced arthritis can be suppressed by the simultaneous inhibition of TNF-alpha, PARP, and NADPH oxidase.

引用

页码：203 / 215

页数：13

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