Nonrandom Distribution of Pseudomonas aeruginosa and Staphylococcus aureus in Chronic Wounds

被引:367
作者
Fazli, Mustafa [1 ]
Bjarnsholt, Thomas [1 ]
Kirketerp-Moller, Klaus [2 ]
Jorgensen, Bo [2 ]
Andersen, Anders Schou [2 ,3 ]
Krogfelt, Karen A. [3 ]
Givskov, Michael [1 ]
Tolker-Nielsen, Tim [1 ]
机构
[1] Univ Copenhagen, Fac Hlth Sci, Dept Int Hlth Immunol & Microbiol, DK-2200 Copenhagen N, Denmark
[2] Bispebjerg Hosp, Copenhagen Wound Healing Ctr, DK-2400 Copenhagen, Denmark
[3] Statens Serum Inst, ABMP, DK-2300 Copenhagen, Denmark
关键词
VENOUS LEG ULCERS; POLYMORPHONUCLEAR LEUKOCYTES; MULTICELLULAR STRUCTURES; EXTRACELLULAR DNA; BIOFILM FORMATION; IV PILI; COLONIZATION; EVASION;
D O I
10.1128/JCM.01395-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The spatial organization of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds was investigated in the present study. Wound biopsy specimens were obtained from patients diagnosed as having chronic venous leg ulcers, and bacterial aggregates in these wounds were detected and located by the use of peptide nucleic acid-based fluorescence in situ hybridization and confocal laser scanning microscopy (CLSM). We acquired CLSM images of multiple regions in multiple sections cut from five wounds containing P. aeruginosa and five wounds containing S. aureus and measured the distance of the bacterial aggregates to the wound surface. The distance of the P. aeruginosa aggregates to the wound surface was significantly greater than that of the S. aureus aggregates, suggesting that the distribution of the bacteria in the chronic wounds was nonrandom. The results are discussed in relation to our recent finding that swab culturing techniques may underestimate the presence of P. aeruginosa in chronic wounds and in relation to the hypothesis that P. aeruginosa bacteria located in the deeper regions of chronic wounds may play an important role in keeping the wounds arrested in a stage dominated by inflammatory processes.
引用
收藏
页码:4084 / 4089
页数:6
相关论文
共 26 条
[1]  
Ågren MS, 2000, ACTA DERM-VENEREOL, P3
[2]  
Andersen A, 2007, J Wound Care, V16, P171
[3]   Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms [J].
Barken, Kim B. ;
Pamp, Suenje J. ;
Yang, Liang ;
Gjermansen, Morten ;
Bertrand, Jacob J. ;
Klausen, Mikkel ;
Givskov, Michael ;
Whitchurch, Cynthia B. ;
Engel, Joanne N. ;
Tolker-Nielsen, Tim .
ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (09) :2331-2343
[4]   Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent [J].
Bjarnsholt, T ;
Jensen, PO ;
Burmolle, M ;
Hentzer, M ;
Haagensen, JAJ ;
Hougen, HP ;
Calum, H ;
Madsen, KG ;
Moser, C ;
Molin, S ;
Hoiby, N ;
Givskov, M .
MICROBIOLOGY-SGM, 2005, 151 :373-383
[5]   Why chronic wounds will not heal: a novel hypothesis [J].
Bjarnsholt, Thomas ;
Kirketerp-Moller, Klaus ;
Jensen, Peter Ostrup ;
Madsen, Kit G. ;
Phipps, Richard ;
Krogfelt, Karen ;
Hoiby, Niels ;
Givskov, Michael .
WOUND REPAIR AND REGENERATION, 2008, 16 (01) :2-10
[6]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322
[7]   MICROBIAL BIOFILMS [J].
COSTERTON, JW ;
LEWANDOWSKI, Z ;
CALDWELL, DE ;
KORBER, DR ;
LAPPINSCOTT, HM .
ANNUAL REVIEW OF MICROBIOLOGY, 1995, 49 :711-745
[8]   Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers [J].
Davies, CE ;
Hill, KE ;
Wilson, MJ ;
Stephens, P ;
Hill, CM ;
Harding, KG ;
Thomas, DW .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3549-3557
[9]   Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo [J].
Davis, Stephen C. ;
Ricotti, Carlos ;
Cazzaniga, Alex ;
Welsh, Esperanza ;
Eaglstein, William H. ;
Mertz, Patricia M. .
WOUND REPAIR AND REGENERATION, 2008, 16 (01) :23-29
[10]   Bacterial evasion of innate host defenses -: the Staphylococcus aureus lesson [J].
Fedtke, I ;
Götz, F ;
Peschel, A .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 294 (2-3) :189-194