Induction of macrophage inflammatory protein MIP-1 alpha mRNA on glial cells after focal cerebral ischemia in the rat

被引：57

作者：

Takami, S

Nishikawa, H

Minami, M

Nishiyori, A

Sato, M

Akaike, A

Satoh, M

机构：

[1] KYOTO UNIV,DEPT MOL PHARMACOL,FAC PHARMACEUT SCI,KYOTO 60601,JAPAN

[2] KYOTO UNIV,DEPT PHARMACOL,FAC PHARMACEUT SCI,KYOTO 60601,JAPAN

来源：

NEUROSCIENCE LETTERS | 1997年 / 227卷 / 03期

关键词：

macrophage inflammatory protein-1 alpha; microglia; astrocyte; ischemia; middle cerebral artery occlusion; double in situ hybridization; rat; HEAT-SHOCK PROTEIN; MESSENGER-RNA; MOLECULAR-CLONING; EXPRESSION; PROTEIN-1-ALPHA;

D O I：

10.1016/S0304-3940(97)00338-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The distribution and cell source of macrophage inflammatory protein-1 alpha (MIP-1 alpha) mRNA induced by transient and permanent middle cerebral artery occlusion (MCAO) were investigated by a double in situ hybridization technique. The distribution and time course of the induction of MIP-1 alpha mRNA were similar in the two MCAO models. MIP-1 alpha mRNA was not detected in the sham-operated rat brain. MIP-1 alpha mRNA was induced by MCAO with the peak of expression at 4-6 h after the onset of occlusion, and the signals of MIP-1 alpha mRNA were observed in the ischemic core region at an earlier time point, and thereafter intensely in the penumbra of the ischemic area. The signals of MIP-1 alpha mRNA were evident on Mac-1 alpha mRNA-positive cells, but not on glial fibrillary acidic protein (GFAP) mRNA-positive cells, indicating that MIP-1 alpha mRNA was induced in microglia/macrophages of the rat brain after focal cerebral ischemia. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：173 / 176

页数：4

共 12 条

[1] EXPRESSION AND REGULATION OF HUMAN NEUTROPHIL-DERIVED MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA [J].