Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells

被引：42

作者：

Creixell, Mar ^{[1
,2
]}

Herrera, Adriana P. ^{[1
]}

Ayala, Vanessa ^{[1
]}

Latorre-Esteves, Magda ^{[1
]}

Perez-Torres, Marianela ^{[3
]}

Torres-Lugo, Madeline ^{[1
]}

Rinaldi, Carlos ^{[1
]}

机构：

[1] Univ Puerto Rico, Dept Chem Engn, Mayaguez, PR 00681 USA

[2] Univ Barcelona, Fac Phys, Dept Elect, E-08028 Barcelona, Spain

[3] Univ Puerto Rico, Dept Pharmaceut Sci, San Juan, PR 00936 USA

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2010年 / 322卷 / 15期

基金：

美国国家科学基金会;

关键词：

Magnetic nanoparticle; Carboxymethyldextran; Epidermal growth factor (EGF); Epidermal growth factor receptor (EGFR); Active targeting; MONODISPERSE MAGNETITE NANOPARTICLES; IN-VIVO; COPOLYMER MICELLES; DRUG-DELIVERY; PERMEABILITY; DIAGNOSIS;

D O I：

10.1016/j.jmmm.2010.02.019

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Epidermal growth factor (EGF) was conjugated with carboxymethyldextran (CMDx) coated iron oxide magnetic nanoparticles using carbodiimide chemistry to obtain magnetic nanoparticles that target the epidermal growth factor receptor (EGFR). Epidermal growth factor modified magnetic nanoparticles were colloidally stable when suspended in biological buffers such as PBS and cell culture media. Both targeted and non-targeted nanoparticles were incubated with CaCo-2 cancer cells, known to overexpress EGFR. Nanoparticle localization within the cell was visualized by confocal laser scanning microscopy and light microscopy using Prussian blue stain. Results showed that targeted magnetic nanoparticles were rapidly accumulated in both flask-shaped small vesicles and large circular endocytic structures. Internalization patterns suggest that both clathrin-dependent and clathrin-independent receptors mediated endocytosis mechanisms are responsible for nanoparticle internalization. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2244 / 2250

页数：7

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