The Ig mutator is dependent on the presence, position, and orientation of the large intron enhancer

被引：45

作者：

Bachl, J

Olsson, C

Chitkara, N

Wabl, M ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[2] Basel Inst Immunol, CH-4005 Basel, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 05期

关键词：

hypermutation; transfected target sequence; termination codon; reversion;

D O I：

10.1073/pnas.95.5.2396

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hypermutation at the Ig loci is confined to the area between the promoter and the intronic enhancer, which includes the rearranged variable region gene segment. We identified factors that contribute to the site-specificity at the heavy chain locus, We found that distance from both the promoter and the intronic enhancer is crucial in hypermutation. The presence of the enhancer is required, and, in contrast to its definition for transcriptional activity, its effect is orientation-sensitive.

引用

页码：2396 / 2399

页数：4

共 36 条

[1] An immunoglobulin mutator that targets G center dot C base pairs [J].