Crystallization and preliminary X-ray analysis of the Clostridium thermocellum cellulosome xylanase Z feruloyl esterase domain

被引：6

作者：

Blum, DL ^{[1
]}

Schubot, FD ^{[1
]}

Ljungdahl, LG ^{[1
]}

Rose, JP ^{[1
]}

Wang, BC ^{[1
]}

机构：

[1] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900006521

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Feruloyl esterases cleave ferulic acid from arabinoxylan and pectin. Feruloyl groups are believed to crosslink the polysaccharide chain within the polymer and to link hemicellulose to lignin, which may play a role in controlling the growth of plants. The Clostridium thermocellum cellulosome xylanase Z feruloyl esterase was expressed in Escherichia coli, purified and crystallized. The crystals diffract to 2.4 Angstrom resolution and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 43.14, b = 63.77, c = 79.57 Angstrom. Assuming one molecule per asymmetric unit, the Matthews coefficient is calculated to be 1.87 Angstrom(3) Da(-1), which corresponds to a solvent content of 34%.

引用

页码：1027 / 1029

页数：3

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