Cloning, sequencing and expression in Escherichia coli of the primary alcohol dehydrogenase gene from Thermoanaerobacter ethanolicus JW200

被引：4

作者：

Holt, PJ ^{[1
]}

Williams, RE ^{[1
]}

Jordan, KN ^{[1
]}

Lowe, CR ^{[1
]}

Bruce, NC ^{[1
]}

机构：

[1] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 190卷 / 01期

关键词：

type III alcohol dehydrogenase; thermostable alcohol dehydrogenase; Thermoanaerobacter ethanolicus;

D O I：

10.1111/j.1574-6968.2000.tb09262.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The structural gene, adhA, for a thermostable primary alcohol dehydrogenase was cloned from Thermoanaerobacter ethanolicus JW200. Constitutive expression from its own promoter was observed in Escherichia coli. The nucleotide sequence of adhA corresponded to an open reading frame of 1197 bp, encoding a polypeptide of 399 amino acids with a calculated M-r of 43 192. Amino acid sequence analysis showed 67-69% identity with alcohol dehydrogenases from two archaeal species and 29-37% identity with bacterial type III alcohol dehydrogenases. This represents the first reported cloning of an alcohol dehydrogenase from a bacterial species that is both thermostable and active against primary long-chain alcohols. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：57 / 62

页数：6

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