Chemiluminescent-based methods to detect subpicomole levels of c-type cytochromes

被引：78

作者：

Feissner, R ^{[1
]}

Xiang, YB ^{[1
]}

Kranz, RG ^{[1
]}

机构：

[1] Washington Univ, Dept Biol, St Louis, MO 63130 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 315卷 / 01期

关键词：

heme protein; detection; chemiluminescence; cytochrome c;

D O I：

10.1016/S0003-2697(02)00658-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of luminol-based substrates and either an autoradiographic film or a charge-coupled device (CCD) imaging system were used for chemiluminescence detection of c-type cytochromes. The Pierce Femto peroxidase substrate was at least 10 times more sensitive when using film than the highly cited 3,3',5,5'-tetramethylbenzidine (benzidine derivative) staining method and 50 times more sensitive when using a CCD imaging system. Film or CCD imaging result in highly sensitive and quantitative signals. The quantitative detection of c-type cytochromes from single colonies or from less than 1 ml of a bacterial culture is possible. (C) 2003 Elsevier Science (USA). All rights reserved.

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页码：90 / 94

页数：5

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