Attenuation of nociceptin/orphanin FQ-induced signaling by N-methyl-D-aspartate in neuronal cells

被引:21
作者
Zhao, J
Zhang, Y
Xin, SM
Ma, L
Pei, G
机构
[1] Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China
[2] Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai 200031, Peoples R China
[3] Shanghai Med Univ, Natl Lab Med Neurobiol, Shanghai 200032, Peoples R China
关键词
cyclic AMP assay; G protein activation; NMDA; nociceptin orphanin FQ (N/OFQ); ORL1; signaling modulation;
D O I
10.1097/00001756-199803090-00013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
ACUTE incubation of NMDA with neuroblastoma x glioma hybrid (NG108-15) cells or neuroblastoma SK-N-SH cells produced significant attenuation of nociceptin/orphanin FQ (N/OFQ)-induced activation of G protein and inhibition of adenylyl cyclase. The attenuation of N/OFQ signaling by NMDA was dose-dependent, blockable by NMDA antagonists, and not observed in cells lacking NMDA receptors, indicating that the effect of NMDA is mediated by the NMDA receptor. Furthermore, NMDA antagonist pretreatment greatly attenuated N/OFQ-induced acute homologous desensitization of ORL1. Interestingly, the signaling induced by etorphine, an opioid agonist of wide spectrum, was sensitive to NMDA treatment in NG108-15 but insensitive in SK-N-SH cells, suggesting differential modulation of opioid signaling by NMDA. The attenuation effects of NMDA on mu opioid receptor-mediated signaling were also observed.
引用
收藏
页码:631 / 636
页数:6
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