Structural characterization and anti-inflammatory activity of two water-soluble polysaccharides from Bellamya purificata

Two water-soluble polysaccharides of Bellamya punficata named BPS-1 and BPS-2 were isolated from the foot muscle of B. purificata, purified by anion-exchange, gel-permeation chromatography and their anti-inflammatory activity was tested. They were homogenous with a molecular weight of 7.2 x 10(6) Da and 8.3 x 10(6) Da by HPGPC, respectively. BPS-1 was composed of glucose, fucose, arabiinose and xylose with a molar ratio of 99:2:1:1, it has a backbone of alpha-(1 -> 4)-linked glucose residues, with branches at C-6 consisting of terminal and alpha-(1 -> 3)-linked glucose residues: BPS-2 was composed of glucose. fucose, arabinose, galactose, xylsoe and mannose with a molar ratio of 218:5:3:2:1:1, and it has a backbone of alpha-(1 -> 4)-linked glucose residues, with branches at C-3 and C-6 consisting of terminal and alpha-(1 -> 3)-linked glucose residues. Anti-inflammatory activity experiments showed that BPS-1 and BPS-2 possessed potent inhibiting functions on mouse ear edema induced by xylene, with the inhibition rate of 57.56% and 56.46% at close 1 mg/mL. respectively. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.