Two-dimensional map of basic proteins of Haemophilus influenzae

被引：34

作者：

Fountoulakis, M

Takács, B

Langen, H

机构：

[1] F Hoffmann La Roche & Co Ltd, Preclin Cent Nervous Syst Res Gene Technol, Div Pharma, CH-4070 Basel, Switzerland

[2] F Hoffmann La Roche & Co Ltd, Infect Dis, Div Pharma, CH-4070 Basel, Switzerland

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 05期

关键词：

two-dimensional protein map; two-dimensional polyacrylamide gel electrophoresis; basic proteins; Haemophilus influenzae;

D O I：

10.1002/elps.1150190527

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Haemophilus influenzae is a bacterium of medical interest of which the entire genome has been sequenced. The proteome of the microorganism has been analyzed by two-dimensional gel electrophoresis, during which immobilized pH 3-10 gradient strips were used and approximately 300 proteins were identified. In order to detect additional, basic proteins, we analyzed the soluble protein fraction of H. influenzae and the proteins of fractions collected from affinity chromatography on heparin, by two-dimensional gel electrophoresis, using for the first-dimensional separation immobilized pH gradient strips comprising the pH region of 6-11. The protein spots were analyzed by matrix-assisted laser desorption ionization-mass spectrometry. One hundred and two proteins were identified, of which 58 were identified for the first time. A large percentage of the basic proteins represent nucleic acid binding and, in particular, ribosomal proteins. The locations of the identified basic proteins of H. influenzae are indicated in a two-dimensional map.

引用

页码：761 / 766

页数：6

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