Glycogen synthase kinase 3 has a proapoptotic function in Hydra gametogenesis

被引:24
作者
Rentzsch, F
Hobmayer, B
Holstein, TW
机构
[1] Univ Heidelberg, Dept Mol Evolut & Genom, D-69120 Heidelberg, Germany
[2] Univ Innsbruck, Inst Zool & Limnol, Ctr Mol Biosci, A-6020 Innsbruck, Austria
[3] Max Planck Inst Immunobiol, Freiburg, Germany
[4] Tech Univ Darmstadt, Inst Zool, D-64287 Darmstadt, Germany
关键词
Cnidaria; Hydra; GSK3; Wnt; oogenesis; gametogenesis; apoptosis; nurse cell;
D O I
10.1016/j.ydbio.2004.10.007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In an approach to study the evolutionary conservation of molecules of the Wnt signal transduction pathway, we analyzed the function of the major negative regulator of this pathway, GSK3 (glycogen synthase kinase 3), in the basal metazoan Hydra. Microinjection assays reveal that HyGSK3 inhibits beta-catenin in zebrafish embryos, indicating that the function of GSK3 in the canonical Wnt signaling pathway is evolutionarily conserved. In Hydra, HyGSK3 transcripts are strongly upregulated during gametogenesis. Treatment of female polyps with the GSK3 inhibitors lithium and alsterpaullone prevents the differentiation of nurse cells and subsequent oocyte formation. Our data indicate that GSK3 is required for the early induction of apoptosis in germline cells, which has been shown to be an initial step in Hydra gametogenesis. Our experiments show that main functions of GSK3 are evolutionarily conserved and unique to multicellular animals, a conclusion which is additionally supported by the presence of specific regulatory domains in the HyGSK3 protein. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:1 / 12
页数:12
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