NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1

被引:31
作者
Daines, DA
Wright, LF
Chaffin, DO
Rubens, CE
Silver, RP
机构
[1] Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14642 USA
[2] Univ Washington, Childrens Hosp, Dept Pediat, Seattle, WA 98105 USA
[3] Reg Med Ctr, Seattle, WA 98105 USA
关键词
Escherichia coli; K1; capsule; NeuD; sialic acid; group B Streptococcus;
D O I
10.1111/j.1574-6968.2000.tb09244.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from region 2 is involved in sialic acid synthesis. A non-polar chromosomal deletion in neuD was constructed. The defect was complemented by neuD in trans or by the addition of exogenous sialic acid. A NeuD homologue, Neu(III)D, from serotype III Streptococcus agalactiae (GBS) also restored capsule expression to the neuD deletion strain. These data confirm the role of neuD in E. coli sialic acid capsule synthesis and demonstrate that the neu(III)D homologue from GBS shares a similar enzymatic function. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
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页码:281 / 284
页数:4
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