Group a rotavirus in sewage samples from Barcelona and Cairo:: Emergence of unusual genotypes

被引:88
作者
Villena, C [1 ]
El-Senousy, WM [1 ]
Abad, FX [1 ]
Pintó, RM [1 ]
Bosch, A [1 ]
机构
[1] Univ Barcelona, Dept Microbiol, Enter Virus Grp, E-08028 Barcelona, Spain
关键词
D O I
10.1128/AEM.69.7.3919-3923.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The presence of rotavirus strains in sewage samples from Cairo, Egypt (November 1998 to October 1999), and Barcelona, Spain (November 1998 to December 2002), was investigated by using a generic molecular detection method based on amplification of a VP6 gene fragment. Overall, 85.7 and 66.9% of the sewage samples from Cairo and Barcelona, respectively, were positive. Positive samples were characterized further, and VP7 and VP4 genotypes were determined. Although 30% of the positive samples from Cairo were G untypeable, the distribution of G types in the positive samples was 69.6% G1, 13% G3, 8.7% G4, and 8.7% G9. The percentage of untypeable samples was much higher for the Barcelona samples (56.5%), and the distribution in the positive samples was 56.4% G1, 3 1.5% G3, 6% G9, 4% G2, and 2% G5. When the P types were examined, 26.7% of the positive samples from Cairo were untypeable, and the distribution of types in the positive samples was 53.3% P[8], 30% P[6], and 16.6% P[4]. In Barcelona, 27.2% of the samples were P untypeable, and the frequencies of the types detected were 49.7% P[8], 37.2% P[4], 8.8% P[6], and 4.2% P[9]. The distribution for strains from Cairo was 38.5% P[8]G1, 27% P[6]G1, 11.5% P[4]G1, 11.5% P[8]G3,7.7% P[6]G4, and 3.8% P[8]G9. Strikingly, equivalent frequencies of common and uncommon strains were observed for Barcelona samples, and the distribution was 38.8% P[8]G1, 30.6% P[4]G1, 11.6% P[8]G3,6.6% P[4]G3,5.8% P[6]G1, 1.6% P[6]G3,1.6% P[9]G1, 0.8% P[41G2, 0.8% P[61G9, 0.8% P[8]G9, and 0.8% P[8]G5. Additionally, two P[-]G5 strains were isolated in Barcelona, and the porcine or human origin of these strains was unclear. Rotavirus variability exhibited not only a geographic pattern but also a temporal pattern.
引用
收藏
页码:3919 / 3923
页数:5
相关论文
共 36 条
[1]   Rotavirus strain diversity in Rio de Janeiro, Brazil:: Characterization of VP4 and VP7 genotypes in hospitalized children [J].
Araújo, IT ;
Fialho, AM ;
de Assis, RMS ;
Rocha, M ;
Galvao, M ;
Cruz, CM ;
Ferreira, MSR ;
Leite, JPG .
JOURNAL OF TROPICAL PEDIATRICS, 2002, 48 (04) :214-218
[2]   Rotavirus genotypes P[4]G9, P[6]G9, and P[8]G9 in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil [J].
Araújo, IT ;
Ferreira, MSR ;
Fialho, AM ;
Assis, RM ;
Cruz, CM ;
Rocha, M ;
Leite, JPG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1999-2001
[3]  
Argüelles MH, 2000, J CLIN MICROBIOL, V38, P252
[4]   Surveillance for rotavirus in Argentina [J].
Bok, K ;
Castagnaro, N ;
Borsa, A ;
Nates, S ;
Espul, C ;
Fay, O ;
Fabri, A ;
Grinstein, S ;
Miceli, I ;
Matson, DO ;
Gómez, JA .
JOURNAL OF MEDICAL VIROLOGY, 2001, 65 (01) :190-198
[5]   RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].
BOOM, R ;
SOL, CJA ;
SALIMANS, MMM ;
JANSEN, CL ;
WERTHEIMVANDILLEN, PME ;
VANDERNOORDAA, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503
[6]  
BOSCH A, 1988, ZBL HYG UMWELTMED, V186, P273
[7]   VP7 and VP4 genotypes among rotavirus strains recovered from children with gastroenteritis over a 3-year period in Valencia, Spain [J].
Buesa, J ;
de Souza, CO ;
Asensi, M ;
Martínez, C ;
Prat, J ;
Gil, MT .
EUROPEAN JOURNAL OF EPIDEMIOLOGY, 2000, 16 (06) :501-506
[8]   ROTAVIRUS ISOLATE WI61 REPRESENTING A PRESUMPTIVE NEW HUMAN SEROTYPE [J].
CLARK, HF ;
HOSHINO, Y ;
BELL, LM ;
GROFF, J ;
HESS, G ;
BACHMAN, P ;
OFFIT, PA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (09) :1757-1762
[9]  
Cubitt WD, 2000, J MED VIROL, V61, P150, DOI 10.1002/(SICI)1096-9071(200005)61:1&lt
[10]  
150::AID-JMV24&gt