Development of a scintillation proximity assay for peroxisome proliferator-activated receptor γ ligand binding domain

被引：93

作者：

Nichols, JS

Parks, DJ

Gonsler, TG

Blanchard, SG ^{[1
]}

机构：

[1] Glaxo Wellcome Inc, Dept Mol Biochem, Res Triangle Pk, NC 27709 USA

[2] Glaxo Wellcome Inc, Dept Mol Sci, Res Triangle Pk, NC 27709 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 257卷 / 02期

关键词：

D O I：

10.1006/abio.1997.2557

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain is described, Scintillation proximity offers an equilibrium method for detecting ligands that is both cost effective and fully automatable. The method described here is the first reported scintillation proximity assay for a peroxisome proliferator-activated receptor. The design of this system is generic in nature, allowing it to be adapted for other ligand binding proteins. (C) 1998 Academic Press.

引用

页码：112 / 119

页数：8

共 19 条

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