Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana

被引：14

作者：

Chung, Ho Yong ^{[1
]}

Fujioka, Shozo ^{[2
]}

Choe, Sunghwa ^{[3
]}

Lee, Soyoun ^{[1
]}

Lee, Youn Hyung ^{[4
]}

Baek, Nam In ^{[1
]}

Chung, In Sik ^{[1
]}

机构：

[1] Kyung Hee Univ, Dept Genet Engn, Plant Metab Res Ctr, Yongin 446701, South Korea

[2] RIKEN, Adv Sci Inst, Wako, Saitama 3510198, Japan

[3] Seoul Natl Univ, Dept Biol Sci, Coll Nat Sci, Seoul 151747, South Korea

[4] Kyung Hee Univ, Dept Hort, Yongin 446701, South Korea

来源：

PLANT CELL REPORTS | 2010年 / 29卷 / 04期

关键词：

Arabidopsis thaliana; Brassinosteroid; Campesterol; Sterol biosynthesis; Suppression; RNA interference; GROWTH; CYTOCHROME-P450; PHYTOSTEROLS; BRASSINOLIDE; METABOLISM; OXIDATION; MECHANISM; CYP85A2;

D O I：

10.1007/s00299-010-0830-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We generated transgenic lines of Arabidopsis thaliana with an RNA interference construct that expressed hairpin double-stranded RNA for DET2:DWF4:SMT2 to induce sequence-specific RNA silencing. In transgenic plants, expressions of DET2, DWF4, and SMT2 were simultaneously reduced, and the campesterol content was increased by up to 420% compared to the level in the wild-type plant. Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates. Transgenic plants harboring the RNA interference construct displayed a semi-dwarf phenotype due to altered development. Our findings indicate that redesigning of plant architecture is possible through simultaneous suppression of multiple genes involved in BR biosynthesis.

引用

页码：397 / 402

页数：6

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