Variation of polymorphic region C of the protein A gene during persistent airway infection of cystic fibrosis patients reflects two independent mechanisms of genetic change in Staphylococcus aureus

被引：83

作者：

Kahl, BC

Mellmann, A

Deiwick, S

Peters, G

Harmsen, D

机构：

[1] Univ Munster, inst Med Microbiol, D-48149 Munster, Germany

[2] Univ Munster, Inst Hyg, D-48149 Munster, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 01期

关键词：

D O I：

10.1128/JCM.43.1.502-505.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Variation of the polymorphic region of the protein A gene (spa) was observed during long-term persistence of Staphylococcus aureus in the airways of 10 cystic fibrosis patients and occurred at a rate of one genetic change every 70 months. Independent mutational events were observed eight times in 142 isolates: four deletions, two duplications of repeats, and two point mutations.

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页码：502 / 505

页数：4

相关论文

共 12 条

[1] NUCLEOTIDE-SEQUENCE OF A VARIANT PROTEIN-A OF STAPHYLOCOCCUS-AUREUS SUGGESTS MOLECULAR HETEROGENEITY AMONG STRAINS [J].

BRIGIDO, MD ;

BARARDI, CRM ;

BONJARDIN, CA ;

SANTOS, CLS ;

JUNQUEIRA, MD ;

BRENTANI, RR .

JOURNAL OF BASIC MICROBIOLOGY, 1991, 31 (05) :337-345

[2] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus [J].

Enright, MC ;

Day, NPJ ;

Davies, CE ;

Peacock, SJ ;

Spratt, BG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) :1008-1015

[3]

Frenay H. M., 1996, EUR J CLIN MICROBIOL, V15, P768

[4] Increased frequency of genomic alterations in Staphylococcus aureus during chronic infection is in part due to phage mobilization [J].

Goerke, C ;

Papenberg, SMY ;

Dasbach, S ;

Dietz, K ;

Ziebach, R ;

Kahl, BC ;

Wolz, C .

JOURNAL OF INFECTIOUS DISEASES, 2004, 189 (04) :724-734

[5] Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management [J].

Harmsen, D ;

Claus, H ;

Witte, W ;

Rothgänger, J ;

Claus, H ;

Turnwald, D ;

Vogel, U .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) :5442-5448

[6] Population dynamics of persistent Staphylococcus aureus isolated from the airways of cystic fibrosis patients during a 6-year prospective study [J].

Kahl, BC ;

Duebbers, A ;

Lubritz, G ;

Haeberle, J ;

Koch, HG ;

Ritzerfeld, B ;

Reilly, M ;

Harms, E ;

Proctor, RA ;

Herrmann, M ;

Peters, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) :4424-4427

[7] spa Typing method for discriminating among Staphylococcus aureus isolates:: Implications for use of a single marker to detect genetic micro- and macrovariation [J].

Koreen, L ;

Ramaswamy, SV ;

Graviss, EA ;

Naidich, S ;

Musser, JA ;

Kreiswirth, BN .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (02) :792-799

[8] Variable numbers of tandem repeat loci in genetically homogeneous Haemophilus influenzae strains alter during persistent colonisation of cystic fibrosis patients [J].

Renders, N ;

Licciardello, L ;

Ijsseldijk, C ;

Sijmons, M ;

van Alphen, L ;

Verbrugh, H ;

van Belkum, A .

FEMS MICROBIOLOGY LETTERS, 1999, 173 (01) :95-102

[9] Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains [J].

Shopsin, B ;

Gomez, M ;

Montgomery, SO ;

Smith, DH ;

Waddington, M ;

Dodge, DE ;

Bost, DA ;

Riehman, M ;

Naidich, S ;

Kreiswirth, BN .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) :3556-3563

[10] INTERPRETING CHROMOSOMAL DNA RESTRICTION PATTERNS PRODUCED BY PULSED-FIELD GEL-ELECTROPHORESIS - CRITERIA FOR BACTERIAL STRAIN TYPING [J].

TENOVER, FC ;

ARBEIT, RD ;

GOERING, RV ;

MICKELSEN, PA ;

MURRAY, BE ;

PERSING, DH ;

SWAMINATHAN, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (09) :2233-2239