Assay of T- and NK-cell subsets and the expression of NKG2A and NKG2D in patients with new-onset systemic lupus erythematosus

被引：58

作者：

Li, Wen-Xian ^{[1
]}

Pan, Hai-Feng ^{[1
]}

Hu, Jian-Li ^{[1
]}

Wang, Chang-Zhong ^{[1
,2
]}

Zhang, Ning ^{[1
]}

Li, Jing ^{[1
]}

Li, Xiang-Pei ^{[3
]}

Xu, Jian-Hua ^{[4
]}

Ye, Dong-Qing ^{[1
]}

机构：

[1] Anhui Med Univ, Dept Epidemiol & Biostat, Sch Publ Hlth, Hefei 230032, Anhui, Peoples R China

[2] Anhui Univ Tradit Chinese Med, Dept Immunol, Hefei, Peoples R China

[3] Anhui Prov Hosp, Dept Rheumatol, Hefei, Peoples R China

[4] Anhui Med Univ, Affiliated Hosp 1, Dept Rheumatol, Hefei, Peoples R China

来源：

CLINICAL RHEUMATOLOGY | 2010年 / 29卷 / 03期

基金：

中国国家自然科学基金;

关键词：

NK-cell subset; NKG2A; NKG2D; Systemic lupus erythematosus; T-cell subset; NATURAL-KILLER-CELLS; WHITE BLOOD-CELLS; LYMPHOCYTE SUBSETS; KILLING DEFECT; DISEASE; QUANTIFICATION; AUTOANTIBODIES; CYTOTOXICITY; INDUCTION; TOLERANCE;

D O I：

10.1007/s10067-009-1322-9

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

This study aims to explore the percentage of T-cell and NK-cell subsets, the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells on the total lymphocytes in new-onset systemic lupus erythematosus (SLE) patients, and explore clinical significance of these cell subsets. Thirty-two SLE patients and 32 normal controls were enrolled. Flow cytometry was used to count T- and NK-cell subsets and to detect the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells in patients with new-onset SLE. Results show that CD4+ T (t = 2.04, P < 0.05), CD4+/CD8+ T cell (t = 2.66, P < 0.05), CD4+ CD25+ T (t = 2.48, P < 0.05), CD3+CD56+ natural killer T (NKT) (t = 40.05, P < 0.01), CD3-CD56+CD16+ NK-cell subsets (t = 3.50, P < 0.01) were significantly decreased, CD8+ T-cell subsets was significantly increased in patients with new-onset SLE (t = 3.80, P < 0.01), as compared with healthy controls. CD8+ T-cell subset was significantly increased in patients with vasculitis (t = 2.47, P < 0.05), and CD3-CD56+CD16+ NK was increased in patients with arthritis (t = 3.21, P < 0.01). However, no statistically significant correlation was found among different PBMC subsets and SLEDAI activity scores. Patients with SLE had a lower expression of NKG2A (U = 2.42, P < 0.05) as well as NKG2A/NKG2D ratio (t = 2.61, P < 0.05) and a higher expression of NKG2D (t = 2.21, P < 0.05) on CD3+ T cells, compared with normal controls. However, they had a higher expression of NKG2A (t = 2.59, P < 0.05) as well as NKG2A/NKG2D ratio (t = 49.45, P < 0.01) and a lower expression of NKG2D (t = 3.05, P < 0.01) on CD3-CD56+ NK cells. Taken together, the findings indicate the decreased CD4+ T-cell, CD4+/CD8+ T-cell, CD4+CD25+ T-cell, CD3+CD56+ NKT-, and CD3-CD56+CD16+ NK-cell subsets, increased CD8+ T-cell subsets as well as the abnormal expression of NKG2A and NKG2D on CD3+ T and CD3-CD56 + NK cells may play a role in the etiology of SLE.

引用

页码：315 / 323

页数：9

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