Pulcherosine, an oxidatively coupled trimer of tyrosine in plant cell walls: Its role in cross-link formation

被引:63
作者
Brady, JD
Sadler, IH
Fry, SC
机构
[1] Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh Cell Wall Grp, Edinburgh EH9 3JH, Midlothian, Scotland
[2] Univ Edinburgh, Dept Chem, Edinburgh EH9 3JH, Midlothian, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
Lycopersicon sp; Solanaceae; tomato; cell cultures; isodityrosine; pulcherosine; di-isodityrosine; extensin; oxidative phenolic coupling; tyrosine; inter-polypeptide cross-linking; plant cell walls;
D O I
10.1016/S0031-9422(97)00592-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An oxidatively coupled trimer of tyrosine has been isolated from hydrolysates of primary cell walls of a tomato cell culture. UV-absorption, fluorescence and H-1 NMR spectra showed that the trimer was pulcherosine, composed of isodityrosine and tyrosine oxidatively coupled via a biphenyl linkage such that the aromatic core is 2,2'-dihydroxy-3-phenoxybiphenyl. Pulcherosine could act as an intermediate in the conversion of isodityrosine to the tetramer, di-isodityrosine. Steric considerations show that the three tyrosine units of pulcherosine could not be near-neighbour residues within a single polypeptide chain. Pulcherosine therefore forms inter-polypeptide cross-links and/or wide intra-polypeptide loops. (C) 1997 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:349 / 353
页数:5
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