Determination of Deoxynivalenol in Medicinal Herbs and Related Products by GC-ECD and Confirmation by GC-MS

被引：48

作者：

Yue, Yan-Tao ^{[1
,2
]}

Zhang, Xiao-Fei ^{[1
,2
]}

Pan, Jianyu ^{[1
]}

Zhen Ou-Yang ^{[2
]}

Wu, Jun ^{[3
]}

Yang, Mei-Hua ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

[2] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

[3] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China

来源：

CHROMATOGRAPHIA | 2010年 / 71卷 / 5-6期

关键词：

Gas chromatography; Derivatization; Medicinal herbs and related products; Deoxynivalenol; CHROMATOGRAPHY-MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; TRICHOTHECENE MYCOTOXINS; LIQUID-CHROMATOGRAPHY; FUSARIUM TOXINS; B-TRICHOTHECENE; LC-MS/MS; VOMITOXIN; WHEAT; QUANTIFICATION;

D O I：

10.1365/s10337-010-1477-1

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

GC with electron capture detection has been applied for the determination of deoxynivalenol (DON) in medicinal herbs and related products. The method consists of extracting the sample with water followed by clean-up of the resulting extract with an immunoaffinity column. DON was determined as its heptofluorobuturyl ester. Positive results were further confirmed by GC-MS. Recoveries from different medicinal herbs spiked with DON at levels ranging from 100 to 5,000 mu g kg(-1) were from 85.5 to 97.2%, with relative standard deviations (RSDs) being less than 6.3%. The limit of detection was 2.0 mu g kg(-1). Out of a total of 58 samples, DON was only found at low concentrations in three samples, ranging from 17.2 to 50.5 mu g kg(-1). This is the first report of DON determination and contamination in medicinal herbs and related products in China.

引用

页码：533 / 538

页数：6

共 29 条

[1]

Rapid simultaneous determination of major type A- and B-trichothecenes as well as zearalenone in maize by high performance liquid chromatography-tandem mass spectrometry [J].