Multiplex polymerase chain reaction for identification and differentiation of Streptococcus equi subsp zooepidemicus and Streptococcus equi subsp equi

被引：52

作者：

Alber, J ^{[1
]}

El-Sayed, A ^{[1
]}

Lämmler, C ^{[1
]}

Hassan, AA ^{[1
]}

Weiss, R ^{[1
]}

Zschöck, M ^{[1
]}

机构：

[1] Univ Giessen, Inst Pharmakol & Toxikol, D-35392 Giessen, Germany

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2004年 / 51卷 / 10期

关键词：

D O I：

10.1111/j.1439-0450.2004.00799.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The closely related streptococcal species Streptococcus equi subsp. zooepidemicus and S. equi subsp. equi were identified by polymerase chain reaction using oligomicleotide primers designed according to species-specific parts of the superoxide dismutase A encoding gene sodA. A further differentiation of both subspecies could be performed by amplification of the genes seeH and seeI encoding the exotoxins SeeH and SeeI, respectively, which could be detected for S. equi subsp. equi but not for S. equi subsp. zooepidemicus. A further simplification of the identification and differentiation of both subspecies was conducted by sodA-seeI multiplex polymerase chain reaction.

引用

页码：455 / 458

页数：4

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