The ontogeny of soleus muscles in mdx and wild type mice

The satellite cell, the organotypic muscle stem cell. is the key element in ontogenetic and load induced muscle fibre growth and repair. It is therefore possible that the satellite pool becomes exhausted with age, especially in mdx mice where dystrophin deficiency results in skeletal muscle degeneration. We compared structural criteria and satellite cell frequencies in soleus muscles of 26 mdx and 23 wild type mice aged aged between 26 and 720 days. The total number of muscle fibres was similar in both groups and remained stable throughout life. except for an early increase in wild type mice. However. in mdx muscles there was always a proportion of small-diameter fibres which resulted in a reduction in the effective myogenic area on cross-section, whereas total cross-sectional area and muscle weights were increased relative to controls throughout life. In adult animals, the frequency and numbers of satellite cells remained stable with age and were similar in both animal groups. Satellite cell numbers showed some considerable variation between individual animals. although with a markedly smaller variability between results of the same animal, pointing to the satellite cell pool being an individual variant. (C) 2004 Published by Elsevier B.V.