Identification and Characterization of Rat RhoA Gene Promoter

被引：8

作者：

Chiba, Yoshihiko ^{[1
]}

Goto, Kumiko ^{[1
]}

Matsusue, Kimihiko ^{[2
]}

Kimura, Shioko ^{[3
]}

Misawa, Miwa ^{[1
]}

机构：

[1] Hoshi Univ, Sch Pharm, Dept Pharmacol, Tokyo 1428501, Japan

[2] Fukuoka Univ, Fac Pharmaceut Sci, Fukuoka 8140180, Japan

[3] NCI, Lab Metab, NIH, Bethesda, MD 20814 USA

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 112卷 / 04期

关键词：

RhoA; transcriptional regulation; bronchial smooth muscle; BRONCHIAL SMOOTH-MUSCLE; MEDIATED CA2+ SENSITIZATION; INHIBITOR; ASTHMA; INTERLEUKIN-13; KINASE; CELLS; JAK2;

D O I：

10.1254/jphs.09346SC

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5'-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13) stimulated cells revealed a significant promoter activity at 238- to 166-bp upstream of the transcription-initiation site, which contains a signal transducer and activation of transcription (STAT) 6 binding region. The IL-13 induced increase in luciferase activity was inhibited by a STAT6 inhibitor, AS 1517499, or a Janus kinases (JAKs) inhibitor, JAK Inhibitor-1, but not by tyrphostin-AG490, WHI-P131, or tyrphostin-AG9 (selective JAK2, JAK3, and Tyk2 inhibitors, respectively). Thus, rat BSM rhoA expression may have causal relation to the IL-13-JAK1-STAT6 signaling.

引用

页码：467 / 472

页数：6

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