Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design

被引：15

作者：

Boltz, Valerie F. ^{[2
]}

Maldarelli, Frank ^{[2
]}

Martinson, Neil ^{[3
,5
]}

Morris, Lynn ^{[4
]}

McIntyre, James A. ^{[3
]}

Gray, Glenda ^{[3
]}

Hopley, Mark J. ^{[6
]}

Kimura, Toshio ^{[6
]}

Mayers, Douglas L. ^{[7
,8
]}

Robinson, Patrick ^{[7
]}

Mellors, John W. ^{[9
]}

Coffin, John M. ^{[2
,10
]}

Palmer, Sarah E. ^{[1
,2
]}

机构：

[1] Karolinska Inst, Dept Virol, Swedish Inst Infect Dis & Control, S-17182 Solna, Sweden

[2] NCI, HIV Drug Resistance Program, NIH, Frederick, MD 21701 USA

[3] Univ Witwatersrand, Perinatal HIV Res Unit, Johannesburg, South Africa

[4] Natl Inst Communicable Dis, Johannesburg, South Africa

[5] Johns Hopkins Univ, Sch Med, Baltimore, MD USA

[6] Boehringer Ingelheim GmbH & Co KG, Ingelheim, Germany

[7] Boehringer Ingelheim Pharmaceut, Ridgefield, CT USA

[8] Idenix Pharmaceut, Cambridge, MA USA

[9] Univ Pittsburgh, Pittsburgh, PA USA

[10] Tufts Univ, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 164卷 / 1-2期

基金：

瑞典研究理事会;

关键词：

HIV-1; Allele-specific PCR; Low frequency variants; Subtype consensus sequence; Patient-specific HIV-1 consensus sequence; IMMUNODEFICIENCY-VIRUS TYPE-1; REVERSE-TRANSCRIPTASE INHIBITORS; SINGLE-DOSE NEVIRAPINE; RESISTANT HIV-1; ANTIRETROVIRAL THERAPY; DRUG-RESISTANCE; TRANSMISSION; POPULATIONS; PERSISTENCE; MUTATIONS;

D O I：

10.1016/j.jviromet.2009.11.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：122 / 126

页数：5

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