Basic fibroblast growth factor enhances PPARγ ligand-induced adipogenesis of mesenchymal stem cells

被引:92
作者
Neubauer, M [1 ]
Fischbach, C [1 ]
Bauer-Kreisel, P [1 ]
Lieb, E [1 ]
Hacker, M [1 ]
Tessmar, J [1 ]
Schulz, MB [1 ]
Goepferich, A [1 ]
Blunk, T [1 ]
机构
[1] Univ Regensburg, Dept Pharmaceut Technol, D-93040 Regensburg, Germany
来源
FEBS LETTERS | 2004年 / 577卷 / 1-2期
关键词
cell differentiation; adipogenesis; basic fibroblast; growth factor; mesenchymal stem cell; peroxisome proliferator-activated receptor gamma; thiazolidinedione;
D O I
10.1016/j.febslet.2004.10.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mesenchymal stem cells (MSCs) are capable of differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth and differentiation factors. It is widely acknowledged that basic fibroblast growth factor (bFGF) modulates chondrogenic and osteogenic differentiation of MSCs, but thorough investigations of its effects on adipogenic differentiation are lacking. In this study, we demonstrate on the cellular and molecular level that supplementation of bFGF in different phases of cell culture leads to a strong enhancement of adipogenesis of MSCs, as induced by an adipogenic hormonal cocktail. In cultures receiving bFGF, mRNA expression of peroxisome proliferator-activated receptor gamma2 (PPARgamma2), a key transcription factor in adipogenesis, was upregulated even prior to adipogenic induction. In order to investigate the effects of bFGF on PPARgamma ligand-induced adipogenic differentiation, the thiazolidinedione troglitazone was administered as a single adipogenic inducer. Basic FGF was demonstrated to also strongly increase adipogenesis induced by troglitazone, that is, bFGF clearly increased the responsiveness of MSCs to a PPARgamma ligand. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:277 / 283
页数:7
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