The next ice age: cryo-electron tomography of intact cells

被引：51

作者：

Steven, AC ^{[1
]}

Aebi, U

机构：

[1] NIAMSD, Struct Biol Lab, NIH, Bethesda, MD 20892 USA

[2] Univ Basel, Bioctr, ME Muller Inst Struct Biol, CH-4056 Basel, Switzerland

来源：

TRENDS IN CELL BIOLOGY | 2003年 / 13卷 / 03期

关键词：

D O I：

10.1016/S0962-8924(03)00023-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent advances in electron tomography are beginning to reveal the internal structure of eukaryotic cells in their native states in three dimensions at molecular resolution. These observations represent the culmination of years of effort to develop protocols for automated data collection, image reconstruction and cryogenic preservation. Cryo-tomograms of Dictyostelium cells depict distinct populations of ribosomes, proteasomes and networks of actin filaments interconnected by branching or bundling, apparently controlled by strategically placed actin-associated proteins.

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页码：107 / 110

页数：4

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