Characterization of the 5′ region of the rat brain glycine transporter GLYT2 gene:: identification of a novel isoform

被引：51

作者：

Ponce, J ^{[1
]}

Poyatos, I ^{[1
]}

Aragón, C ^{[1
]}

Giménez, C ^{[1
]}

Zafra, F ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Ciencias, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain

来源：

NEUROSCIENCE LETTERS | 1998年 / 242卷 / 01期

关键词：

glycine; transporter; gene organization;

D O I：

10.1016/S0304-3940(98)00037-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have identified two alternative 5' ends for the GLYT2 glycine transporter in rat brain DNA by using rapid amplification of cDNA ends (RACE) analysis. Study of the genomic DNA revealed that the isoform diversity is generated by alternative use of exons Ia or Ib, respectively. Upon translation, the mRNA corresponding to the novel isoform GLYT2b would yield a protein five amino acids longer than the previously characterized isoform GLYT2a. Both forms display similar regional distribution and kinetics characteristics. However, whereas GLYT2a is able to actively accumulate glycine into transfected COS cells, GLYT2b seems only to exchange (or release) glycine. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：25 / 28

页数：4

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