DNA of Drosophila melanogaster contains 5-methylcytosine

被引：148

作者：

Gowher, H

Leismann, O

Jeltsch, A

机构：

[1] Inst Biochem, Fachbereich 8, D-35392 Giessen, Germany

[2] Univ Bayreuth, Lehrstuhl Genet, D-95440 Bayreuth, Germany

来源：

EMBO JOURNAL | 2000年 / 19卷 / 24期

关键词：

DNA methylation; DNA methyltransferase; DNA modification; Drosophila melanogaster; 5-methylcytosine;

D O I：

10.1093/emboj/19.24.6918

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [gamma-P-32]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D.melanogaster at a level of similar to1 in 1000-2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development.

引用

页码：6918 / 6923

页数：6

共 54 条

[1] ESTIMATION OF THE AMOUNT OF 5-METHYLCYTOSINE IN DROSOPHILA-MELANOGASTER DNA BY AMPLIFIED ELISA AND PHOTOACOUSTIC-SPECTROSCOPY [J].