Molecular cloning, characterisation and tissue-specific expression of human LAG3, a member of the novel Lag1 protein family

被引：16

作者：

Cai, XF ^{[1
]}

Tao, Z ^{[1
]}

Yan, ZQ ^{[1
]}

Yang, SL ^{[1
]}

Gong, Y ^{[1
]}

机构：

[1] Chinese Acad Sci, SIBS, Shanghai Res Ctr Biotechnol, Shanghai 200233, Peoples R China

来源：

DNA SEQUENCE | 2003年 / 14卷 / 02期

关键词：

LAG3Hs; membrane protein; Lag1 protein family; Saccharomyces cerevisiae;

D O I：

10.1080/1042517021000041831

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We identified and characterised the cDNA coding for human Lag3, a member of the novel eukaryotic Lag1 protein family which was related to the Saccharomyces cerevisiae longevity-assurance gene LAG1. The composite nucleotide sequence revealed a coding region of 1140 bp, a 5'-UTR of 210 by and a 3'-UTR of 900 bp. The deduced sequence of 380 amino acids had six transmembrane regions and the conserved Lag1 motif. Northern blot analysis revealed that the similar to2.2 kb LAG3Hs transcript was expressed in all 12 human tissues, high in kidney and liver, moderate in brain, heart, skeletal muscle, placenta and lung and low in colon, thymus, spleen, small intestine and peripheral blood leukocyte. Because of its structural similarity to LAG1, it eras postulated that LAG3Hs may mediate sphingolipiids metabolism.

引用

页码：79 / 86

页数：8

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