Transient marker stabilisation: a general procedure to construct marker-free recombinant vaccinia virus

被引：18

作者：

Scheiflinger, F ^{[1
]}

Dorner, F ^{[1
]}

Falkner, FG ^{[1
]}

机构：

[1] Immuno AG, Biomed Res Ctr, A-2304 Orth Donau, Austria

来源：

ARCHIVES OF VIROLOGY | 1998年 / 143卷 / 03期

关键词：

D O I：

10.1007/s007050050303

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant vaccinia viruses based on the highly attenuated Modified Vaccinia Ankara (MVA) strain expressing HIV-1 antigen genes were constructed by a novel procedure involving the transient use of two marker genes. The selectable markers used, the Escherichia coli guanine phosphoribosyltransferase (gpt) and the beta-galactosidase (lacZ) genes, are not retained within the final recombinant virus. The transient marker stabilisation (TMS) procedure allows the generation of marker-free recombinant viruses in a series of simple plaque purification steps. HIV-1 gag pol genes were inserted into two loci of vaccinia MVA demonstrating the efficiency of the procedure.

引用

页码：467 / 474

页数：8

共 25 条

[1] Characterization of the vaccinia MVA hemagglutinin gene locus and its evaluation as an insertion site for foreign genes [J].

Antoine, G ;

Scheiflinger, F ;

Holzer, G ;

Langmann, T ;

Falkner, FG ;

Dorner, F .

GENE, 1996, 177 (1-2) :43-46

[2] SELECTION OF RECOMBINANT VACCINIA VIRUSES ON THE BASIS OF PLAQUE-FORMATION [J].

BLASCO, R ;

MOSS, B .

GENE, 1995, 158 (02) :157-162

[3] A DOMINANT SELECTABLE MARKER FOR THE CONSTRUCTION OF RECOMBINANT POXVIRUSES [J].

BOYLE, DB ;

COUPAR, BEH .

GENE, 1988, 65 (01) :123-128

[4]

CARROLL MW, 1995, BIOTECHNIQUES, V19, P352

[5]

CARROLL MW, 1995, BIOTECHNIQUES, V19, P356

[6] VACCINIA VIRUS EXPRESSION VECTOR - COEXPRESSION OF BETA-GALACTOSIDASE PROVIDES VISUAL SCREENING OF RECOMBINANT VIRUS PLAQUES [J].

CHAKRABARTI, S ;

BRECHLING, K ;

MOSS, B .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3403-3409

[7] ESCHERICHIA-COLI GPT GENE PROVIDES DOMINANT SELECTION FOR VACCINIA VIRUS OPEN READING FRAME EXPRESSION VECTORS [J].

FALKNER, FG ;

MOSS, B .

JOURNAL OF VIROLOGY, 1988, 62 (06) :1849-1854

[8] TRANSIENT DOMINANT SELECTION OF RECOMBINANT VACCINIA VIRUSES [J].

FALKNER, FG ;

MOSS, B .

JOURNAL OF VIROLOGY, 1990, 64 (06) :3108-3111

[9] NEOMYCIN RESISTANCE AS A DOMINANT SELECTABLE MARKER FOR SELECTION AND ISOLATION OF VACCINIA VIRUS RECOMBINANTS [J].

FRANKE, CA ;

RICE, CM ;

STRAUSS, JH ;

HRUBY, DE .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) :1918-1924

[10]

GELICH C, 1994, EXPRESSION GAG POL P

← 1 2 3 →