Ascorbate oxidase based amperometric biosensor for organophosphorous pesticide monitoring

被引：40

作者：

Rekha, K ^{[1
]}

Gouda, MD ^{[1
]}

Thakur, MS ^{[1
]}

Karanth, NG ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Fermentat Technol & Bioengn Dept, Mysore 570013, Karnataka, India

来源：

BIOSENSORS & BIOELECTRONICS | 2000年 / 15卷 / 9-10期

关键词：

ascorbate oxidase; organophosphorous pesticide; amperometry; biosensor;

D O I：

10.1016/S0956-5663(00)00077-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An amperometric principle based biosensor containing tissues of cucumber, rich in ascorbic acid oxidase, was used for the detection of organophosphorous (OP) pesticide ethyl paraoxon, which inhibits the activity of ascorbic acid oxidase. The optimal concentration of ascorbic acid used as substrate was found to be 5.67 mM. The biosensor response was found to reach steady state within 2 min. A measurable inhibition (>10%) was obtained with 10 min incubation of the enzyme electrode with different concentrations of the pesticide. There was a linear relationship between the percentage of inhibition of the enzyme substrate reaction and the pesticide (ethyl paraoxon) concentration in the range 1-10 ppm with a regression value 0.9942. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：499 / 502

页数：4

共 11 条

[1] CHEMILUMINESCENCE-BASED INHIBITION-KINETICS OF ALKALINE-PHOSPHATASE IN THE DEVELOPMENT OF A PESTICIDE BIOSENSOR [J].

AYYAGARI, MS ;

KAMTEKAR, S ;

PANDE, R ;

MARX, KA ;

KUMAR, J ;

TRIPATHY, SK ;

AKKARA, J ;

KAPLAN, DL .

BIOTECHNOLOGY PROGRESS, 1995, 11 (06) :699-703

[2]

COURY AL, 1989, APPL BIOSENSORS, P7

[3] Increased paraoxon detection with solvents using acetylcholinesterase inactivation measured with a choline oxidase biosensor [J].

Fennouh, S ;

Casimiri, V ;

Burstein, C .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (02) :97-104

[4]

GALLO MA, 1991, HDB PESTICIDE TOXICO, V2, P5

[5] A dual enzyme amperometric biosensor for monitoring organophosphorous pesticides [J].

Gouda, MD ;

Thakur, MS ;

Karanth, NG .

BIOTECHNOLOGY TECHNIQUES, 1997, 11 (09) :653-655

[6]

Hartley Ian C., 1994, Analytical Proceedings, V31, P333, DOI 10.1039/ai9943100333

[7] POTENTIOMETRIC BUTYRYLCHOLINE SENSOR FOR ORGANOPHOSPHATE PESTICIDES [J].

IMATO, T ;

ISHIBASHI, N .

BIOSENSORS & BIOELECTRONICS, 1995, 10 (05) :435-441

[8] DETERMINATION OF ORGANOPHOSPHOROUS AND CARBAMATE INSECTICIDES BY FLOW-INJECTION ANALYSIS [J].

KUMARAN, S ;

TRANMINH, C .

ANALYTICAL BIOCHEMISTRY, 1992, 200 (01) :187-194

[9] DETERMINATION OF ORGANOPHOSPHORUS INSECTICIDES WITH A CHOLINE ELECTROCHEMICAL BIOSENSOR [J].

PALLESCHI, G ;

BERNABEI, M ;

CREMISINI, C ;

MASCINI, M .

SENSORS AND ACTUATORS B-CHEMICAL, 1992, 7 (1-3) :513-517

[10]

SCHELLER F, 1992, BIOSENSORS, P24

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