Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/Pi-IIb) gene promoter and characterization of gene structure

被引：7

作者：

Arima, K ^{[1
]}

Collins, JF ^{[1
]}

Hines, ER ^{[1
]}

Bai, LQ ^{[1
]}

Ghishan, FK ^{[1
]}

机构：

[1] Univ Arizona, Hlth Sci Ctr, Steele Mem Childrens Res Ctr, Dept Pediat & Physiol, Tucson, AZ 85724 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1494卷 / 1-2期

关键词：

sodium-phosphate transporter; promoter analysis; intestine;

D O I：

10.1016/S0167-4781(00)00205-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of the murine Na/P-i-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. (C) 2000 Elsevier Science B.V. All rights reserved.

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页码：149 / 154

页数：6

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