Rejoining of DNA by the RAG1 and RAG2 proteins

被引：88

作者：

Melek, M

Gellert, M ^{[1
]}

van Gent, DC

机构：

[1] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

[2] Erasmus Univ, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

来源：

SCIENCE | 1998年 / 280卷 / 5361期

关键词：

D O I：

10.1126/science.280.5361.301

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assembly of immunoglobulin and T cell receptor genes from separate gene segments [V(D)J recombination] begins with DNA double-strand breakage by the RAG1 and RAG2 proteins, acting at a pair of recombination signal sequences (RSSs). Here, the RAG proteins are shown to reverse the cleavage reaction by joining an RSS to a broken coding sequence end. These "hybrid joints" have also been found in lymphoid cells, even when the normal pathway of DNA double-strand break repair is inactive, and can now be explained by this activity of the RAG proteins.

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收藏

页码：301 / 303

页数：3

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