Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

被引：7

作者：

Blanco, LP ^{[1
]}

Toro, CS ^{[1
]}

Romero, JM ^{[1
]}

Santiviago, CA ^{[1
]}

Mora, GC ^{[1
]}

机构：

[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago, Chile

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 12期

关键词：

Salmonella typhi OmpC; typhoid patients; mononuclear cells; macrophage activation;

D O I：

10.1111/j.1348-0421.1997.tb01960.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro, Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC, The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity, In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells, This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.

引用

页码：999 / 1003

页数：5

共 35 条

[1]

Abbas AK, 1991, CELLULAR MOL IMMUNOL, P226

[2]

BARNIER RA, 1992, ADV MODERN BIOTECHNO

[3]

BLANCO F, 1993, SCAND J INFECT DIS, V25, P73, DOI 10.3109/00365549309169673

[4]

CALDERON I, 1986, INFECT IMMUN, V52, P209

[5] THE HEMOLYTIC EFFECT OF SALMONELLA-TYPHI TY-2 PORINS [J].

CALDERON, I ;

LOBOS, SR ;

MORA, GC .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 141 (03) :579-583

[6]

CHATFIELD S, 1992, SYMPOSIA SOC GENERAL, V49, P299

[7]

COLLINS FM, 1968, J IMMUNOL, V101, P830

[8]

EDELMAN R, 1986, REV INFECT DIS, V8, P329

[9] RELEASE OF CYTOKINES INDUCED BY SALMONELLA-TYPHIMURIUM PORINS [J].

GALDIERO, F ;

DELERO, GC ;

BENEDETTO, N ;

GALDIERO, M ;

TUFANO, MA .

INFECTION AND IMMUNITY, 1993, 61 (01) :155-161

[10] ISOLATION AND CHARACTERIZATION OF GAL-E MUTANT TY 21A OF SALMONELLA-TYPHI - CANDIDATE STRAIN FOR A LIVE, ORAL TYPHOID VACCINE [J].

GERMANIER, R ;

FURER, E .

JOURNAL OF INFECTIOUS DISEASES, 1975, 131 (05) :553-558

← 1 2 3 4 →