Metal-assisted assembly and stabilization of collagen-like triple helices

被引：48

作者：

Cai, WB ^{[1
]}

Kwok, SW ^{[1
]}

Taulane, JP ^{[1
]}

Goodman, M ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 46期

关键词：

D O I：

10.1021/ja0442062

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-chain and TRIS-assembled collagen mimetic peptide structures incorporating catechol groups were synthesized. When 1/3 equiv of Fe3+ was added to the single-chain compound in 50 mM CAPS buffer (pH 10), the 1:3 Fe3+-catechol complex that formed acted as an N-terminal scaffold to assemble the triple helix. When 1 equiv of Fe3+ was added to the TRIS-assembled compound in the buffer solution, the Fe3+-catechol complex acted as an extra C-terminal scaffold, which lead to a triple helix with both termini tethered. The formation of this C-terminal complex increased the Tm by a remarkable 22 °C! Copyright © 2004 American Chemical Society.

引用

页码：15030 / 15031

页数：2

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