Apomorphine up-regulates NGF and GDNF synthesis in cultured mouse astrocytes

被引：62

作者：

Ohta, M ^{[1
]}

Mizuta, I

Ohta, K

Nishimura, M

Mizuta, E

Hayashi, K

Kuno, S

机构：

[1] Natl Utano Hosp, Clin Res Ctr, Ukyo Ku, Kyoto 6068255, Japan

[2] Kobe Pharmaceut Univ, Dept Clin Chem, Kobe, Hyogo 6588558, Japan

[3] Japan Fdn Aging & Hlth, Aichi 4702101, Japan

[4] Gifu Pharmaceut Univ, Gifu 5028585, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 272卷 / 01期

关键词：

apomorphine; dopamine agonist; NGF; GDNF; BDNF; astrocytes; Parkinson's disease; neuroprotection;

D O I：

10.1006/bbrc.2000.2732

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apomorphine, a D-1/D-2 dopamine agonist, is an antiparkinsonian drug. me examined the effects of apomorphine on synthesis of neurotrophic factors in cultured mouse astrocytes. After 24 h incubation with apomorphine, NGF and GDNF contents in the culture medium increased to 122-fold and 1.8-fold of the control, respectively; whereas the BDNF content did not change significantly. In Northern blot analysis, expression of NGF mRNA in astrocytes reached the maximum level at 6 h after addition of the drug. By semiquantitative RT-PCR analysis, the GDNF transcript level was found to reach 2.8-fold of the control level at 15 h. These results suggest that apomorphine may exert neuroprotective effects by stimulation of NGF and GDNF synthesis in astrocytes. (C) 2000 Academic Press.

引用

页码：18 / 22

页数：5

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