Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal

被引:451
作者
Alves, M
Xiao, LH
Sulaiman, I
Lal, AA
Matos, O
Antunes, F
机构
[1] Univ Nova Lisboa, Unidade Protozoarios Oportunistas VIH & Outras Pr, Inst Higiene & Med Trop, UPMM, P-1349008 Lisbon, Portugal
[2] Univ Lisbon, Fac Med, Hosp Santa Maria, Univ Doencas Infecciosas, P-1600 Lisbon, Portugal
[3] Ctr Dis Control & Prevent, Div Parasit Dis, Atlanta, GA 30341 USA
关键词
D O I
10.1128/JCM.41.6.2744-2747.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.
引用
收藏
页码:2744 / 2747
页数:4
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