Enteric microflora contribute to constitutive ICAM-1 expression on vascular endothelial cells

被引:32
作者
Komatsu, S
Berg, RD
Russell, JM
Nimura, Y
Granger, DN
机构
[1] Louisiana State Univ, Med Ctr, Dept Cellular & Mol Physiol, Shreveport, LA 71130 USA
[2] Louisiana State Univ, Med Ctr, Dept Microbiol & Immunol, Shreveport, LA 71130 USA
[3] Nagoya Univ, Sch Med, Dept Surg 1, Nagoya, Aichi 4668550, Japan
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2000年 / 279卷 / 01期
关键词
germ-free mice; leukocyte-endothelial cell adhesion; inflammation; bacterial translocation; host defense;
D O I
10.1152/ajpgi.2000.279.1.G186
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Quantitative estimates of endothelial cell adhesion molecule expression have revealed that some adhesion molecules [e.g., intercellular adhesion molecule-1 (ICAM-1)] are abundantly expressed in different vascular beds under normal conditions. The objective of this study was to determine whether the enteric microflora contribute to the constitutive expression of ICAM-1 and other endothelial cell adhesion molecules in the gastrointestinal tract and other regional vascular beds. The dual radiolabeled monoclonal antibody technique was used to measure endothelial expression of ICAM-1, ICAM-2, vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in conventional, germfree mice and germ-free mice receiving the cecal contents of conventional mice to reestablish the enteric microflora (total association). Constitutive ICAM-1 expression was significantly lower in the splanchnic organs (pancreas, stomach, small and large intestine, mesentery, and liver), kidneys, skeletal muscle, and skin of germ-free mice compared with their conventional counterparts. These differences were abolished after total association of germ-free mice with the indigenous gastrointestinal flora. The expression of ICAM-2, VCAM-1, and E-selectin in the various tissues studied did not differ between conventional and germ-free mice. These findings indicate that the indigenous gastrointestinal microflora are responsible for a significant proportion of the basal ICAM-1 expression detected in both intestinal and extraintestinal tissues.
引用
收藏
页码:G186 / G191
页数:6
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