A point mutation in the 14α-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus

被引：225

作者：

Diaz-Guerra, TM ^{[1
]}

Mellado, E ^{[1
]}

Cuenca-Estrella, M ^{[1
]}

Rodriguez-Tudela, JL ^{[1
]}

机构：

[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Unidad Micol, Madrid 28220, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 03期

关键词：

D O I：

10.1128/AAC.47.3.1120-1124.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.

引用

页码：1120 / 1124

页数：5

共 27 条

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