Metabolic fingerprinting of Ephedra species using 1H-NMR spectroscopy and principal component analysis

The metabolomic analysis of Ephedra species was performed using H-1-NMR spectroscopy and multivariate data analysis. A broad range of metabolites could be detected by H-1-NMR spectroscopy without any chromatographic separation. The principal component analysis used to reduce the huge data set obtained from the H-1-NMR spectra of the plant extracts clearly discriminated three different Ephedra species. The major differences in Ephedra sinica, Ephedra intermedia and Ephedra distachya var. distachya were found to be due to benzoic acid analogues in the aqueous fraction and ephedrine-type alkaloids in the organic fraction. Based on this metabolomic recognition, one of nine commercial Ephedra materials evaluated was shown to be a mixture of Ephedra species. This method will be a useful tool for chemotaxonomic analysis and authentification of Ephedra species including quality control of plant materials.