Identification and evaluation of antioxidants defatted Camellia oleifera seeds by isopropanol salting-out pretreatment

被引：96

作者：

Chen, Jung-Hui ^{[1
]}

Wu, Hsing-Yu ^{[2
]}

Liau, Bing-Chung ^{[1
]}

Chang, Chieh-Ming J. ^{[4
]}

Jong, Ting-Ting ^{[1
]}

Wu, Li-Chen ^{[2
,3
]}

机构：

[1] Natl Chung Hsing Univ, Dept Chem, Taichung 40227, Taiwan

[2] Natl Chi Nan Univ, Grad Inst Biomed & Biomed Technol, Puli 54561, Nantou County, Taiwan

[3] Natl Chi Nan Univ, Dept Appl Chem, Puli 54561, Nantou County, Taiwan

[4] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 40227, Taiwan

来源：

FOOD CHEMISTRY | 2010年 / 121卷 / 04期

关键词：

Camellia oleifera; Salting-out; TEAC; ORAC; ABTS*; FLUORESCENCE-BASED ASSAY; KAEMPFEROL GLYCOSIDES; FREE-RADICALS; TEA PLANT; FLAVONOIDS; SINENSIS; OLIGOGLYCOSIDES; CONSTITUENTS; CHEMISTRY; ANALOGS;

D O I：

10.1016/j.foodchem.2010.01.015

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Seven flavonoid glycosides (1-7) and one theasaponin (8) were isolated from the defatted Camellia oleifera seeds using the isopropanol salting-out pretreatment (ISP) technique, followed by column chromatography. Among them, compounds 5, 7, and 8 are new compounds. The comparison of oxygen radical absorbance capacity (ORAC) of crude extracts extracted by the n-butanol partition (BP) with those extracted by the ISP showed that the latter method yields eight times greater scavenging capacities than that of the former approach. Besides, ISP exhibited almost twice the extraction efficiency than that of BP. ORAC and Trolox equivalent antioxidant capacity (TEAC) analysis revealed that the most potent antioxidants are compounds 1 and 2, which exhibited 1.78 and 1.41 times higher antioxidant activity than that of Trolox, respectively. These results indicated that the ISP approach improves the extraction efficiency in the isolation of potent antioxidants, making the defatted C. oleifera seeds valuable in the food additive industry. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1246 / 1254

页数：9

共 29 条

[1]

Free radicals, oxidative stress, and antioxidants in human health and disease [J].

Aruoma, OI .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1998, 75 (02) :199-212

[2]

BABA K, 1986, CHEM PHARM BULL, V34, P1540

[3]

Simple coumarins and analogues in medicinal chemistry: Occurrence, synthesis and biological activity [J].

Borges, F ;

Roleira, F ;

Milhazes, N ;

Santana, L ;

Uriarte, E .

CURRENT MEDICINAL CHEMISTRY, 2005, 12 (08) :887-916

[4]

KAEMPFEROL GLYCOSIDES FROM HOSTA-VENTRICOSA [J].

BUDZIANOWSKI, J .

PHYTOCHEMISTRY, 1990, 29 (11) :3643-3647

[5]

Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships [J].

Cao, GH ;

Sofic, E ;

Prior, RL .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :749-760

[6]

Extraction and purification of flavanone glycosides and kaemferol glycosides from defatted Camellia oleifera seeds by salting-out using hydrophilic isopropanol [J].

Chen, Jung-Hui ;

Liau, Bing-Chung ;

Jong, Ting-Ting ;

Chang, Chieh-Ming J. .

SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 67 (01) :31-37

[7]

FLUORESCENCE-BASED ASSAY FOR REACTIVE OXYGEN SPECIES - A PROTECTIVE ROLE FOR CREATININE [J].

GLAZER, AN .

FASEB JOURNAL, 1988, 2 (09) :2487-2491

[8]

GLAZER AN, 1990, METHOD ENZYMOL, V186, P161

[9]

High molecular weight plant polyphenolics (tannins) as biological antioxidants [J].

Hagerman, AE ;

Riedl, KM ;

Jones, GA ;

Sovik, KN ;

Ritchard, NT ;

Hartzfeld, PW ;

Riechel, TL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (05) :1887-1892

[10]

Chemistry of free radicals in lipids [J].

Hamilton, RJ ;

Kalu, C ;

Prisk, E ;

Padley, FB ;

Pierce, H .

FOOD CHEMISTRY, 1997, 60 (02) :193-199

← 1 2 3 →