Paper-based archiving of mammalian and plant samples for RNA analysis

The ability to archive biological samples for subsequent nucleic acid analysis is essential for tissue specimens and forensic samples. FTA(R) Card is a chemically treated filter paper designed for the collection and room temperature storage of biological samples for subsequent DNA analysis Ifs usefulness for the preservation of biological samples for subsequent RNA analysis was rested. Here, we demonstrate that RNA in biological samples stared on FTA Cards is stable and can be used successfully for RT-PCR and northern blot analysis. RNA stability depends on the storage temperature and the type of biological specimen. RNA in mammalian cells stored on FTA Cards is stable for over one year at temperatures at or below -20 degreesC and for five to three months in samples scored at room temperature. For plant leaf longer storage times (> 5 days) require temperatures at or below 70 degreesC following sample application. FTA Cards may constitute a method not only for convenient collection and storage of biological samples but also for rapid RT-PCR analysis of tissue and cell samples.