Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness

被引：31

作者：

Ge, Y

Rajkumar, L

Guzman, RC

Nandi, S

Patton, WF

Agnew, BJ

机构：

[1] Dept Proteom Mol Probres, Eugene, OR USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Canc Res Lab, Berkeley, CA 94720 USA

来源：

PROTEOMICS | 2004年 / 4卷 / 11期

关键词：

breast cancer; electrophoresis; fluorescence; fractionation;

D O I：

10.1002/pmic.200400957

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns.

引用

页码：3464 / 3467

页数：4

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