Signaling mechanism for receptor-activated canonical transient receptor potential 3 (TRPC3) channels

被引:136
作者
Trebak, M [1 ]
Bird, GSJ [1 ]
McKay, RRM [1 ]
Birnbaumer, L [1 ]
Putney, JW [1 ]
机构
[1] NIEHS, NIH, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA
关键词
D O I
10.1074/jbc.M300544200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Canonical transient receptor potential 3 (TRPC3) is a receptor-activated, calcium permeant, non-selective cation channel. TRPC3 has been shown to interact physically with the N-terminal domain of the inositol 1,4,5-trisphosphate receptor, consistent with a "conformational coupling" mechanism for its activation. Here we show that low concentrations of agonists that fail to produce levels of inositol 1,4,5-trisphosphate sufficient to induce Ca2+ release from intracellular stores substantially activate TRPC3. By several experimental approaches, we demonstrate that neither inositol 1,4,5-trisphosphate nor G proteins are required for TRPC3 activation. However, diacylglycerols were sufficient to activate TRPC3 in a protein kinase C-independent manner. Surface receptor agonists and exogenously applied diacylglycerols were not additive in activating TRPC3. In addition, inhibition of metabolism of diacylglycerol slowed the reversal of receptor-dependent TRPC3 activation. We conclude that receptor-mediated activation of phospholipase C in intact cells activates TRPC3 via diacylglycerol production, independently of G proteins, protein kinase C, or inositol 1,4,5-trisphosphate.
引用
收藏
页码:16244 / 16252
页数:9
相关论文
共 37 条
  • [1] An examination of the secretion-like coupling model for the activation of the Ca2+ release-activated Ca2+ current ICRAC in RBL-1 cells
    Bakowski, D
    Glitsch, MD
    Parekh, AB
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2001, 532 (01): : 55 - 71
  • [2] CAPACITATIVE CALCIUM-ENTRY
    BERRIDGE, MJ
    [J]. BIOCHEMICAL JOURNAL, 1995, 312 : 1 - 11
  • [3] ACTIVATION OF CA2+ ENTRY INTO ACINAR-CELLS BY A NON-PHOSPHORYLATABLE INOSITOL TRISPHOSPHATE
    BIRD, GS
    ROSSIER, MF
    HUGHES, AR
    SHEARS, SB
    ARMSTRONG, DL
    PUTNEY, JW
    [J]. NATURE, 1991, 352 (6331) : 162 - 165
  • [4] BIRD GS, 1992, J BIOL CHEM, V267, P17722
  • [5] On the molecular basis and regulation of cellular capacitative calcium entry: Roles for Trp proteins
    Birnbaumer, L
    Zhu, X
    Jiang, MS
    Boulay, G
    Peyton, M
    Vannier, B
    Brown, D
    Platano, D
    Sadeghi, H
    Stefani, E
    Birnbaumer, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (26) : 15195 - 15202
  • [6] Modulation of Ca2+ entry by polypeptides of the inositol 1,4,5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP):: Evidence for roles of TRP and IP3R in store depletion-activated Ca2+ entry
    Boulay, G
    Brown, DM
    Qin, N
    Jiang, MS
    Dietrich, A
    Zhu, MX
    Chen, ZG
    Birnbaumer, M
    Mikoshiba, K
    Birnbaumer, L
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) : 14955 - 14960
  • [7] Stable activation of single Ca2+ release-activated Ca2+ channels in divalent cation-free solutions
    Braun, FJ
    Broad, LM
    Armstrong, DL
    Putney, JW
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) : 1063 - 1070
  • [8] A non-capacitative pathway activated by arachidonic acid is the major Ca2+ entry mechanism in rat A7r5 smooth muscle cells stimulated with low concentrations of vasopressin
    Broad, LM
    Cannon, TR
    Taylor, CW
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1999, 517 (01): : 121 - 134
  • [9] Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry
    Broad, LM
    Braun, FJ
    Lievremont, JP
    Bird, GSJ
    Kurosaki, T
    Putney, JW
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (19) : 15945 - 15952
  • [10] PROTEIN KINASE-C-MEDIATED NEGATIVE-FEEDBACK INHIBITION OF UNSTIMULATED AND BOMBESIN-STIMULATED POLYPHOSPHOINOSITIDE HYDROLYSIS IN SWISS-MOUSE 3T3 CELLS
    BROWN, KD
    BLAKELEY, DM
    HAMON, MH
    LAURIE, MS
    CORPS, AN
    [J]. BIOCHEMICAL JOURNAL, 1987, 245 (03) : 631 - 639