PMMA-based capillary electrophoresis electrochemical detection microchip fabrication

被引：28

作者：

Horng, RH ^{[1
]}

Han, P

Chen, HY

Lin, KW

Tsai, TM

Zen, JM

机构：

[1] Natl Chung Hsing Univ, Inst Precis Engn, Taichung 402, Taiwan

[2] Natl Chung Hsing Univ, Dept Chem, Taichung 402, Taiwan

来源：

JOURNAL OF MICROMECHANICS AND MICROENGINEERING | 2005年 / 15卷 / 01期

关键词：

D O I：

10.1088/0960-1317/15/1/002

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

yIn this paper, a 50 mum (depth) x 50 mum (width) microfluidic channel is made on a poly(methyl methacrylate) (PMMA) substrate using thick photoresist. Openings were drilled for buffer reservoirs on an additional piece of PMMA. A final PMMA/pattemed photoresist/PMMA sandwich configuration was completed using a bonding process. The thick photoresist was used as the adhesion layer and also as the microfluidic system. Using screen-printed technology for carbon and silver electrode fabrication, the microchip electrophoretic device functions were demonstrated. Successful detection of uric acid and L-ascorbic acid (the main components in human urine) validates the functionality of the proposed system. Successful ascorbic and uric acid separation in a sample from a urine donor who had consumed 500 mg of vitamins verified the proposed biochip.

引用

页码：6 / 10

页数：5

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