Lipids are required for directional pollen-tube growth

被引:298
作者
Wolters-Arts, M
Lush, WM
Mariani, C
机构
[1] Catholic Univ Nijmegen, Dept Expt Bot, Grad Sch Expt Plant Sci, NL-6525 ED Nijmegen, Netherlands
[2] Univ Melbourne, Sch Bot, Plant Cell Biol Res Ctr, Parkville, Vic 3052, Australia
关键词
D O I
10.1038/33929
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Successful pollination and fertilization are absolute requirements for sexual reproduction in higher plants. Pollen hydration, germination and penetration of the stigma by pollen tubes are influenced by the exudate on wet stigmas(1) and by the pollen coat in species with dry stigmas(2-5). The exudate allows pollen tubes to grow directly into the stigma, whereas the pollen coat establishes the contact with the stigma. Pollen tubes then grow into the papillae, which are covered by a cuticle. The components of the exudate or pollen coat that are responsible for pollen tube penetration are not known. To discover the role of the exudate, we tested selected compounds for their ability to act as functional substitutes for exudate in the initial stages of pollen-tube growth on transgenic stigmaless tobacco plants' that did not produce exudate. Here we show that lipids are the essential factor needed for pollen tubes to penetrate the stigma, and that, in the presence of these lipids, pollen tubes will also penetrate leaves. We propose that lipids direct pollen-tube growth by controlling the flow of water to pollen in species with dry and wet stigmas.
引用
收藏
页码:818 / 821
页数:4
相关论文
共 30 条
[1]   AXON GUIDANCE BY GRADIENTS OF A TARGET-DERIVED COMPONENT [J].
BAIER, H ;
BONHOEFFER, F .
SCIENCE, 1992, 255 (5043) :472-475
[2]  
Boden N., 1994, MICELLES MEMBRANES M, P153
[3]   Organ-specific and Agamous-regulated expression and glycosylation of a pollen tube growth-promoting protein [J].
Cheung, AY ;
Zhan, XY ;
Wang, H ;
Wu, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :3853-3858
[4]   STIGMA OF NICOTIANA - ULTRASTRUCTURAL AND BIOCHEMICAL-STUDIES [J].
CRESTI, M ;
KEIJZER, CJ ;
TIEZZI, A ;
CIAMPOLINI, F ;
FOCARDI, S .
AMERICAN JOURNAL OF BOTANY, 1986, 73 (12) :1713-1722
[5]  
DICKINSON H, 1995, SEX PLANT REPROD, V8, P1, DOI 10.1007/BF00228756
[6]   PLANT BIOLOGY - POLLEN DRESSED FOR SUCCESS [J].
DICKINSON, H .
NATURE, 1993, 364 (6438) :573-574
[7]   SELF-POLLINATION - SIMPLY A SOCIAL DISEASE [J].
DICKINSON, H .
NATURE, 1994, 367 (6463) :517-518
[8]   LIPOCHEMISTRY OF PROGRAMIC STAGE OF A SELF-INCOMPATIBLE SPECIES - NEUTRAL LIPIDS AND FATTY-ACIDS OF SECRETORY STIGMA DURING ITS GLANDULAR ACTIVITY, AND OF SOLID STYLE, OVARY AND ANTHER IN FORSYTHIA-INTERMEDIA-ZAB (HETEROSTYLIC SPECIES) [J].
DUMAS, C .
PLANTA, 1977, 137 (02) :177-184
[9]   Identification of pollen components regulating pollination-specific responses in the stigmatic papillae of Brassica oleracea [J].
Elleman, CJ ;
Dickinson, HG .
NEW PHYTOLOGIST, 1996, 133 (02) :197-205
[10]   POLLINATION IN SPECIES WITH DRY STIGMAS - THE NATURE OF THE EARLY STIGMATIC RESPONSE AND THE PATHWAY TAKEN BY POLLEN TUBES [J].
ELLEMAN, CJ ;
FRANKLINTONG, V ;
DICKINSON, HG .
NEW PHYTOLOGIST, 1992, 121 (03) :413-424