Adult human colonic subepithelial myofibroblasts express extracellular matrix proteins and cyclooxygenase-1 and -2

被引:134
作者
Mahida, YR [1 ]
Beltinger, J
Makh, S
Göke, M
Gray, T
Podolsky, DK
Hawkey, CJ
机构
[1] Univ Nottingham Hosp, Queens Med Ctr, Div Gastroenterol, Nottingham NG7 2UH, England
[2] Univ Nottingham Hosp, Queens Med Ctr, Div Pathol, Nottingham NG7 2UH, England
[3] Massachusetts Gen Hosp, Gastrointestinal Unit, Boston, MA 02114 USA
[4] Harvard Univ, Sch Med, Boston, MA 02114 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1997年 / 273卷 / 06期
关键词
basement membrane; collagen; laminin; prostaglandin;
D O I
10.1152/ajpgi.1997.273.6.G1341
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Interactions between epithelial cells and subepithelial myofibroblasts are increasingly recognized as important in the regulation of epithelial cell function. We have established primary cultures of subepithelial myofibroblasts from adult human colonic mucosal samples denuded of epithelial cells and maintained in culture. During culture of mucosal tissue, subepithelial myofibroblasts migrated out via basement membrane pores before establishment in culture. Despite prolonged culture and passage, the myofibroblasts maintained their phenotype, as demonstrated by expression of alpha-smooth muscle actin and vimentin. The cells expressed transcripts and protein for cyclooxygenase (COX)-1 and -2 enzymes, and their release of prostaglandin E-2 (PGE(2)) was inhibited by selective COX-1 and -2 inhibitors. The myofibroblasts also expressed the extracellular matrix (ECM) proteins collagen type IV, laminin-beta(1) and -gamma(1), and fibronectin. Adult human colonic subepithelial myofibroblasts may influence epithelial cell function via products of COX-1 and -2 enzymes, such as PGE(2) and secreted ECM proteins.
引用
收藏
页码:G1341 / G1348
页数:8
相关论文
共 31 条