Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli

被引：58

作者：

Montoro, P ^{[1
]}

Teinseree, N

Rattana, W

Kongsawadworakul, P

Michaux-Ferriere, N

机构：

[1] Kasetsart Univ, KAPI, CIRAD, Rubber Project, Bangkok 10900, Thailand

[2] Dept Agr, Bangkok 10900, Thailand

[3] Mahidol Univ, Fac Sci, Dept Biotechnol, Bangkok 10900, Thailand

[4] CIRAD, BIOTROP, F-34032 Montpellier, France

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 09期

关键词：

rubber tree; Agrobacterium tumefaciens; calcium; gene transfer;

D O I：

10.1007/s002990000208

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The influence of CaCl2 was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl2.Five A. tumefaciens strains (C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct further experiments. The calli were precul tured on MM containing a range of CaCl2 concentrations, then inoculated with Agrobacterium suspension. Transfer of friable calli from MM containing 9 mM CaCl2 to calcium-free medium significantly enhanced the transient beta-glucuronidase activity. Interestingly, the use of calcium-free Agrobacterium resuspension medium to inoculate friable calli again dramatically increased the transformation efficiency. Induction of Agrobacterium's virulence with acetosyringone remained an important factor to stimulate transformation.

引用

页码：851 / 855

页数：5

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