A genetically encoded photocaged amino acid

被引：243

作者：

Wu, N

Deiters, A

Cropp, TA

King, D

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 44期

关键词：

D O I：

10.1021/ja040175z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, α-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG. Copyright © 2004 American Chemical Society.

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页码：14306 / 14307

页数：2

相关论文

共 19 条

[1] Site-specific incorporation of a redox-active amino acid into proteins [J].

Alfonta, L ;

Zhang, ZW ;

Uryu, S ;

Loo, JA ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14662-14663

[2] An expanded genetic code with a functional quadruplet codon [J].

Anderson, JC ;

Wu, N ;

Santoro, SW ;

Lakshman, V ;

King, DS ;

Schultz, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7566-7571

[3] RECOGNITION NUCLEOTIDES OF ESCHERICHIA-COLI TRANSFER RNA(LEU) AND ITS ELEMENTS FACILITATING DISCRIMINATION FROM TRANSFER RNA(SER) AND TRANSFER RNA(TYR) [J].

ASAHARA, H ;

HIMENO, H ;

TAMURA, K ;

HASEGAWA, T ;

WATANABE, K ;

SHIMIZU, M .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :219-229

[4] An expanded eukaryotic genetic code [J].

Chin, JW ;

Cropp, TA ;

Anderson, JC ;

Mukherji, M ;

Zhang, ZW ;

Schultz, PG .

SCIENCE, 2003, 301 (5635) :964-967

[5] Progress toward an expanded eukaryotic genetic code [J].

Chin, JW ;

Cropp, TA ;

Chu, S ;

Meggers, E ;

Schultz, PG .

CHEMISTRY & BIOLOGY, 2003, 10 (06) :511-519

[6] Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli [J].

Chin, JW ;

Martin, AB ;

King, DS ;

Wang, L ;

Schultz, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :11020-11024

[7] Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli [J].

Chin, JW ;

Santoro, SW ;

Martin, AB ;

King, DS ;

Wang, L ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (31) :9026-9027

[8] The 2 Å crystal structure of leucyl-tRNA synthetase and its complex with a leucyl-adenylate analogue [J].

Cusack, S ;

Yaremchuk, A ;

Tukalo, M .

EMBO JOURNAL, 2000, 19 (10) :2351-2361

[9] Incorporation of caged cysteine and caged tyrosine into a transmembrane segment of the nicotinic ACh receptor [J].

Philipson, KD ;

Gallivan, JP ;

Brandt, GS ;

Dougherty, DA ;

Lester, HA .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 281 (01) :C195-C206

[10]

Pollitt SK, 1998, ANGEW CHEM INT EDIT, V37, P2104, DOI 10.1002/(SICI)1521-3773(19980817)37:15<2104::AID-ANIE2104>3.0.CO