The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin

被引：100

作者：

Ungewickell, A

Ward, ME

Ungewickell, E

Majerus, PW ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Div Hematol, St Louis, MO 63110 USA

[2] Hannover Med Sch, D-30625 Hannover, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 37期

关键词：

D O I：

10.1073/pnas.0405664101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen 1 endosomal marker in fixed cells. The endosomal localization of Ocrl was confirmed by live-cell time-lapse microscopy in which we monitored the dynamics of Ocrl on endosomes. GST binding assays show that Ocrl interacts with the clathrin terminal domain and the clathrin adaptor protein AP-2. Our findings suggest a role for Ocrl in endosomal receptor trafficking and sorting.

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页码：13501 / 13506

页数：6

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